human bronchial epithelial (hbepic) cells Search Results


human bronchial epithelial cells bepc  (Innoprot Inc)
93
Innoprot Inc human bronchial epithelial cells bepc
Figure 2. Raman imaging of a typical human bronchial <t>epithelial</t> lung cell <t>(BEpC)</t> without (control) and incubated with retinoic acid (50 µM) for 48 h. Microscopy images (A,E), Raman images with the respective Raman spectra (B,D,F,H) of a nucleus (red), lipid droplets (orange), endoplasmic reticulum (blue), cytoplasm (green), mitochondria (magenta), cell border (light grey). Fluorescence images (C,G). Resolution of Raman images 1 µm, integration time 0.3 s, 10 mW at 532 nm and resolution of fluorescence images 1 µm, integration time 0.01 s (Nucleus—blue (Hoechst 33342), ER/lipid droplets—red (Oil Red O)).
Human Bronchial Epithelial Cells Bepc, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+bronchial+epithelial+(hbepic)+cells/Human+Bronchial+Epithelial+Cells/pm37760504-77-11-18
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primary human bronchial epithelial cells  (iXCells Biotechnologies)
91
iXCells Biotechnologies primary human bronchial epithelial cells
Figure 2. Raman imaging of a typical human bronchial <t>epithelial</t> lung cell <t>(BEpC)</t> without (control) and incubated with retinoic acid (50 µM) for 48 h. Microscopy images (A,E), Raman images with the respective Raman spectra (B,D,F,H) of a nucleus (red), lipid droplets (orange), endoplasmic reticulum (blue), cytoplasm (green), mitochondria (magenta), cell border (light grey). Fluorescence images (C,G). Resolution of Raman images 1 µm, integration time 0.3 s, 10 mW at 532 nm and resolution of fluorescence images 1 µm, integration time 0.01 s (Nucleus—blue (Hoechst 33342), ER/lipid droplets—red (Oil Red O)).
Primary Human Bronchial Epithelial Cells, supplied by iXCells Biotechnologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+bronchial+epithelial+(hbepic)+cells/Human+Bronchial+Epithelial+Cells/pm36521489-645-9-15
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primary human bronchial epithelial cells - by Bioz Stars, 2026-10
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human bronchial epithelial cells hbepc  (Cell Applications Inc)
91
Cell Applications Inc human bronchial epithelial cells hbepc
Cell viability ( A ) and cell proliferation ( B ) induced by nicotine in <t>HBEpC</t> and/or si-mRNA-α7-HBEpC viability. ( A ): For cell viability, 7500 cells/cm 2 are plated in T25 flask (total cell number 187,000) and treated with nicotine 1 × 10 −7 M, after 1 h cells are washed three times in PBS Ca 2+ and Mg 2+ free and then incubated in drug-free medium for additionally 48 h. Then, cells (detached or floating) are counted after staining with trypan blue dye. ( B ): For cell proliferation 7500 cells/cm 2 are plated in T25 flask and treated with nicotine every 48 h. Cells are detached and viable cells are counted every 12 h. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction.
Human Bronchial Epithelial Cells Hbepc, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+bronchial+epithelial+(hbepic)+cells/Human+Bronchial+Epithelial+Cells%3A+HBEpC/pmc07794754-196-0-8
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hbepc copd ca502copdk05a cells  (Cell Applications Inc)
90
Cell Applications Inc hbepc copd ca502copdk05a cells
Cell viability ( A ) and cell proliferation ( B ) induced by nicotine in <t>HBEpC</t> and/or si-mRNA-α7-HBEpC viability. ( A ): For cell viability, 7500 cells/cm 2 are plated in T25 flask (total cell number 187,000) and treated with nicotine 1 × 10 −7 M, after 1 h cells are washed three times in PBS Ca 2+ and Mg 2+ free and then incubated in drug-free medium for additionally 48 h. Then, cells (detached or floating) are counted after staining with trypan blue dye. ( B ): For cell proliferation 7500 cells/cm 2 are plated in T25 flask and treated with nicotine every 48 h. Cells are detached and viable cells are counted every 12 h. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction.
Hbepc Copd Ca502copdk05a Cells, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+bronchial+epithelial+(hbepic)+cells/Human+Bronchial+Epithelial+Cells%3A+COPD%3A+HBEpC-COPD/pm33976430-194-7-13
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Image Search Results


Figure 2. Raman imaging of a typical human bronchial epithelial lung cell (BEpC) without (control) and incubated with retinoic acid (50 µM) for 48 h. Microscopy images (A,E), Raman images with the respective Raman spectra (B,D,F,H) of a nucleus (red), lipid droplets (orange), endoplasmic reticulum (blue), cytoplasm (green), mitochondria (magenta), cell border (light grey). Fluorescence images (C,G). Resolution of Raman images 1 µm, integration time 0.3 s, 10 mW at 532 nm and resolution of fluorescence images 1 µm, integration time 0.01 s (Nucleus—blue (Hoechst 33342), ER/lipid droplets—red (Oil Red O)).

Journal: Cancers

Article Title: Control of Mitochondrial Electron Transport Chain Flux and Apoptosis by Retinoic Acid: Raman Imaging In Vitro Human Bronchial and Lung Cancerous Cells.

doi: 10.3390/cancers15184535

Figure Lengend Snippet: Figure 2. Raman imaging of a typical human bronchial epithelial lung cell (BEpC) without (control) and incubated with retinoic acid (50 µM) for 48 h. Microscopy images (A,E), Raman images with the respective Raman spectra (B,D,F,H) of a nucleus (red), lipid droplets (orange), endoplasmic reticulum (blue), cytoplasm (green), mitochondria (magenta), cell border (light grey). Fluorescence images (C,G). Resolution of Raman images 1 µm, integration time 0.3 s, 10 mW at 532 nm and resolution of fluorescence images 1 µm, integration time 0.01 s (Nucleus—blue (Hoechst 33342), ER/lipid droplets—red (Oil Red O)).

Article Snippet: An A549 human lung carcinoma cell line (no. CCL-185, ATCC) and human bronchial epithelial cells (BEpC) (no. P10557, Innoprot) were used.

Techniques: Imaging, Control, Incubation, Microscopy, Fluorescence

Figure 4. Normalized average Raman spectra of mitochondria of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in mitochondria of the BEpC and A549 cells. The concentration c = 0 µM represents the control cells without retinoic acid.

Journal: Cancers

Article Title: Control of Mitochondrial Electron Transport Chain Flux and Apoptosis by Retinoic Acid: Raman Imaging In Vitro Human Bronchial and Lung Cancerous Cells.

doi: 10.3390/cancers15184535

Figure Lengend Snippet: Figure 4. Normalized average Raman spectra of mitochondria of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in mitochondria of the BEpC and A549 cells. The concentration c = 0 µM represents the control cells without retinoic acid.

Article Snippet: An A549 human lung carcinoma cell line (no. CCL-185, ATCC) and human bronchial epithelial cells (BEpC) (no. P10557, Innoprot) were used.

Techniques: Incubation, Concentration Assay, Control

Figure 5. Normalized average Raman spectra of cytoplasm of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in the cytoplasm of the BEpC and A549 cells.

Journal: Cancers

Article Title: Control of Mitochondrial Electron Transport Chain Flux and Apoptosis by Retinoic Acid: Raman Imaging In Vitro Human Bronchial and Lung Cancerous Cells.

doi: 10.3390/cancers15184535

Figure Lengend Snippet: Figure 5. Normalized average Raman spectra of cytoplasm of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in the cytoplasm of the BEpC and A549 cells.

Article Snippet: An A549 human lung carcinoma cell line (no. CCL-185, ATCC) and human bronchial epithelial cells (BEpC) (no. P10557, Innoprot) were used.

Techniques: Incubation, Concentration Assay

Figure 6. Normalized average Raman spectra of lipid droplets of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in mitochondria of the BEpC and A549 cells.

Journal: Cancers

Article Title: Control of Mitochondrial Electron Transport Chain Flux and Apoptosis by Retinoic Acid: Raman Imaging In Vitro Human Bronchial and Lung Cancerous Cells.

doi: 10.3390/cancers15184535

Figure Lengend Snippet: Figure 6. Normalized average Raman spectra of lipid droplets of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in mitochondria of the BEpC and A549 cells.

Article Snippet: An A549 human lung carcinoma cell line (no. CCL-185, ATCC) and human bronchial epithelial cells (BEpC) (no. P10557, Innoprot) were used.

Techniques: Incubation, Concentration Assay

Figure 7. Normalized average Raman spectra of the nucleus of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in the nucleus of the BEpC and A549 cells.

Journal: Cancers

Article Title: Control of Mitochondrial Electron Transport Chain Flux and Apoptosis by Retinoic Acid: Raman Imaging In Vitro Human Bronchial and Lung Cancerous Cells.

doi: 10.3390/cancers15184535

Figure Lengend Snippet: Figure 7. Normalized average Raman spectra of the nucleus of human bronchial (BEpC) (A) and lung cancer cells (A549) (B) cells without and incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h. (C) Raman band ν19 intensity at 1583 cm−1 as a function of retinoic acid concentration in the nucleus of the BEpC and A549 cells.

Article Snippet: An A549 human lung carcinoma cell line (no. CCL-185, ATCC) and human bronchial epithelial cells (BEpC) (no. P10557, Innoprot) were used.

Techniques: Incubation, Concentration Assay

Figure 8. Normalized average Raman spectra of lipid droplets in human bronchial (BEpC) (A) and lung cancer cells (A549) (B) incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h and con- trol (without retinoic acid). (C) Raman band intensity at 2847 cm−1 as a function of retinoic acid concentration in lipid droplets of the BEpC and A549 cells.

Journal: Cancers

Article Title: Control of Mitochondrial Electron Transport Chain Flux and Apoptosis by Retinoic Acid: Raman Imaging In Vitro Human Bronchial and Lung Cancerous Cells.

doi: 10.3390/cancers15184535

Figure Lengend Snippet: Figure 8. Normalized average Raman spectra of lipid droplets in human bronchial (BEpC) (A) and lung cancer cells (A549) (B) incubated with retinoic acid (1, 10, 50 µM) for 24 and 48 h and con- trol (without retinoic acid). (C) Raman band intensity at 2847 cm−1 as a function of retinoic acid concentration in lipid droplets of the BEpC and A549 cells.

Article Snippet: An A549 human lung carcinoma cell line (no. CCL-185, ATCC) and human bronchial epithelial cells (BEpC) (no. P10557, Innoprot) were used.

Techniques: Incubation, Concentration Assay

Cell viability ( A ) and cell proliferation ( B ) induced by nicotine in HBEpC and/or si-mRNA-α7-HBEpC viability. ( A ): For cell viability, 7500 cells/cm 2 are plated in T25 flask (total cell number 187,000) and treated with nicotine 1 × 10 −7 M, after 1 h cells are washed three times in PBS Ca 2+ and Mg 2+ free and then incubated in drug-free medium for additionally 48 h. Then, cells (detached or floating) are counted after staining with trypan blue dye. ( B ): For cell proliferation 7500 cells/cm 2 are plated in T25 flask and treated with nicotine every 48 h. Cells are detached and viable cells are counted every 12 h. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Cell viability ( A ) and cell proliferation ( B ) induced by nicotine in HBEpC and/or si-mRNA-α7-HBEpC viability. ( A ): For cell viability, 7500 cells/cm 2 are plated in T25 flask (total cell number 187,000) and treated with nicotine 1 × 10 −7 M, after 1 h cells are washed three times in PBS Ca 2+ and Mg 2+ free and then incubated in drug-free medium for additionally 48 h. Then, cells (detached or floating) are counted after staining with trypan blue dye. ( B ): For cell proliferation 7500 cells/cm 2 are plated in T25 flask and treated with nicotine every 48 h. Cells are detached and viable cells are counted every 12 h. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Incubation, Staining, Comparison

Expression of Ki67 induced by nicotine in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism; ( C ): Western blotting, ( D ): densitometric analysis. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate. In the , raw data of Western blotting are reported.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Expression of Ki67 induced by nicotine in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism; ( C ): Western blotting, ( D ): densitometric analysis. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate. In the , raw data of Western blotting are reported.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Comparison

SA-β-Gal in HBEpC exposed continuously to nicotine. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: SA-β-Gal in HBEpC exposed continuously to nicotine. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques:

Evaluation of intracellular Ca 2+ after exposure to nicotine for 48 h in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Evaluation of intracellular Ca 2+ after exposure to nicotine for 48 h in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Evaluation of intracellular ATP after exposure to nicotine for 48 h in HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Evaluation of intracellular ATP after exposure to nicotine for 48 h in HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity, performed with Prism. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay

Expression of EGF and p-EGFR after exposure to nicotine for 48 h in HBEpC. ( A ): ELISA experiments for EGFR; ( B ): regression equation linearity for EGFR, performed with Prism; ( C ): ELISA experiments for p-EGFR; ( D ): regression equation linearity for p-EGFR, performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Expression of EGF and p-EGFR after exposure to nicotine for 48 h in HBEpC. ( A ): ELISA experiments for EGFR; ( B ): regression equation linearity for EGFR, performed with Prism; ( C ): ELISA experiments for p-EGFR; ( D ): regression equation linearity for p-EGFR, performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Comparison

Induction of p53 and phospho-p53 induced by nicotine in HBEpC. ( A ): ELISA assay. ( B ): Western blotting experiments, ( C ): densometric analysis. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. In the , raw data of Western blotting are reported.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Induction of p53 and phospho-p53 induced by nicotine in HBEpC. ( A ): ELISA assay. ( B ): Western blotting experiments, ( C ): densometric analysis. Experiments are performed at least two times in triplicate. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. In the , raw data of Western blotting are reported.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Comparison

Induction of phospho-p38 and p38 by nicotine in HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity for phosphor-p38; ( C ): regression equation linearity for phosphor-p38performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Induction of phospho-p38 and p38 by nicotine in HBEpC. ( A ): ELISA experiments; ( B ): regression equation linearity for phosphor-p38; ( C ): regression equation linearity for phosphor-p38performed with Prism. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

EMT induced by nicotine in HBEpC. ( A ): Western blotting; ( B ): densitometric analysis. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate. In the , raw data of Western blotting are reported.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: EMT induced by nicotine in HBEpC. ( A ): Western blotting; ( B ): densitometric analysis. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate. In the , raw data of Western blotting are reported.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Western Blot, Comparison

Cell migration to nicotine for 48 h in HBEpC and/or si-mRNA-α7-HBEpC. HeLa cells are positive control. ( A ) HeLa positive control. ( B ): Cell migration h in HBEpC and/or si-mRNA-α7-HBEpC. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Cell migration to nicotine for 48 h in HBEpC and/or si-mRNA-α7-HBEpC. HeLa cells are positive control. ( A ) HeLa positive control. ( B ): Cell migration h in HBEpC and/or si-mRNA-α7-HBEpC. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Migration, Positive Control, Comparison

Induction of VEGF by nicotine in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ) regression equation linearity for VEGFR. Statistical significance is analyzed with one-way AOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Induction of VEGF by nicotine in HBEpC and/or si-mRNA-α7-HBEpC. ( A ): ELISA experiments; ( B ) regression equation linearity for VEGFR. Statistical significance is analyzed with one-way AOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Anchorage-independent growth induced by nicotine in HBEpC. HeLa cells are positive control and NIH3T3 the negative. ( A ): Representative picture of HBEpC cloned on soft agar. ( B ): Cloned cells. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Anchorage-independent growth induced by nicotine in HBEpC. HeLa cells are positive control and NIH3T3 the negative. ( A ): Representative picture of HBEpC cloned on soft agar. ( B ): Cloned cells. Statistical significance is analyzed with one-way ANOVA with multiple-comparison and post hoc test with Bonferroni correction. Experiments are performed at least two times in triplicate.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Positive Control, Clone Assay, Comparison

Effects induced by nicotine on different pathways in human airway epithelial cells (results obtained in this work and in literature) and comparison with effects caused by SARS-CoV-2, SARS-CoV, MERS-CoV and by non-tumorigenic virus infection on the same pathways.

Journal: Molecules

Article Title: Nicotine Changes Airway Epithelial Phenotype and May Increase the SARS-COV-2 Infection Severity

doi: 10.3390/molecules26010101

Figure Lengend Snippet: Effects induced by nicotine on different pathways in human airway epithelial cells (results obtained in this work and in literature) and comparison with effects caused by SARS-CoV-2, SARS-CoV, MERS-CoV and by non-tumorigenic virus infection on the same pathways.

Article Snippet: Human Bronchial Epithelial Cells (HBEpC) were obtained from Cell Applications Inc. ( www.cellapplications.com/product no. 502K-05a) and cultured in complete Bronchial/Tracheal Epithelial Cell Growth Medium ( www.cellapplications.com/product ) as described previously [ ]. si-mRNA-α7-HBEpC were obtained as described previously [ ].

Techniques: Comparison, Virus, Infection, Expressing, Concentration Assay, In Vitro, Activation Assay, Activity Assay, Knockdown, Control, Migration